Pleurotus pulmonarius LBM 105 metabolome during PCBs degradation
Here we present a metabolomic study of the white rot fungus Pleurotus pulmonarius LBM 105 during the degradation of the Aroclors 1242, 1254 and 1260.
Steps to reproduce
The strain LBM 105 of P. pulmonarius was provided by the Forestry Science Faculty, Universidad Nacional de Misiones, Argentina and was isolated from the Misiones rainforest near the city of Eldorado, Misiones, Argentina. As a PCBs source, a transformer oil containing a mixture of Aroclors 1242, 1254 and 1260 was used, gently donated by Kioshi S.A. (Buenos Aires, Argentina). This oil was first characterized by the “Instituto Nacional de Tecnología Industrial (INTI) - Centro de investigación y Desarrollo de Ambiente” (Buenos Aires, Argentina). All the organic solvents were of p. a. quality, trace analysis quality or gradient grade. The inoculum for the liquid media was obtained from a 5 days old Malt Extract Agar plates (12.7 g/L, malt extract; 20 g/L, agar). The liquid media used in this work was a nitrogen-limited mineral medium (GA) which contained 10 g/L, glucose; 0.5 g/L, asparagine monohydrate; 0.5 g/L, MgSO4.7H2O; 0.5 g/L, H2KPO4; 0.6 g/L, HK2PO4; 0.4 mg/L, CuSO4.5H2O; 0.1 mg/L, MnCl.4H2O; 0.1 mg/L, H3BO3; 0,1 g/L, CaCl2; 0.02 mg/L, Na2MoO4.2H2O; 1 mg/L, FeCl3; 3.5 mg/L, ZnCl2; 0.1 mg/L thiamine hydrochloride; 1.7 mmol/L, Tween 80 to a final pH of 4.6 (Haglund et al., 2002). 20 mL of sterilized GA medium in 250 mL Erlenmeyer flasks were used for the treatment and controls. For the metabolomic study, each Erlenmeyer flask was inoculated with one agar plug (5 mm diameter) of the fungus. The Aroclor mixture was taken from a contaminated transformer oil with a total PCBs concentration of 855 ± 34 mg/g, dissolved in acetone to a final quantity of 4000 μg of total PCBs per flask. The controls consisted in an abiotic culture spiked with the Aroclor mixture. Controls and treatments were carried out in triplicates and incubated at 28 ± 1 °C under static conditions for 28 days. Samples were taken every 7 days and the mycelium was separated from the supernatant by centrifugation (4695×g for 15 min).