Enhanced expression of the myogenic factor Myocyte enhancer factor-2 in imaginal disc myoblasts activates a partial, but incomplete, muscle development program.

Description

This repository contains RNA-seq raw data files for the paper "Enhanced expression of the myogenic factor Myocyte enhancer factor-2 in imaginal disc myoblasts activates a partial, but incomplete, muscle development program."

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Wing imaginal discs were dissected from wandering third instar female larvae and then stored in Qiagen Buffer RLT, at -80C, until a total of 60 wing discs were collected, per replicate. Then, total RNA was extracted from each replicate using RNeasy Micro Kit (#74104; Qiagen, USA). RNA-sequencing (RNA-seq) was conducted using Illumina NovaSeq 6000. Three single-end libraries were made from both control and experimental samples. No trimming was used. The modern aligner HISAT2 was used to map reads to Drosophila reference genome dm6 (D. melanogaster Aug. 2014 BDGP Release 6 + ISO1 MT/dm6) (Kim et al, 2019). Quality control was completed using fastqcr R package. Next, to quantify the expression level of transcripts, the alignment files were used as inputs for featurecounts (Liao et al, 2014). Then, DESeq2 was used to identify significantly expressed genes between the control and experimental samples using these counts (Love et al, 2014). P<0.05 was set as the threshold for significantly differential expressed genes.

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