Viral fitness landscapes in diverse host species reveal multiple evolutionary lines for the NS1 gene of influenza A viruses - Supplemental Data (Part 1)

Published: 22 October 2019| Version 1 | DOI: 10.17632/77x6pyfk79.1
Raquel Munoz-Moreno, Carles Martinez-Romero, Daniel Blanco-Melo, Christian V. Forst, Raffael Nachbagauer, Asiel Arturo Benitez, Nacho Mena, Sadaf Aslam, Vinod Balasubramaniam, Ilseob Lee, Maryline Panis, Juan Ayllon, David Sachs, Man-Seong Park, Florian Krammer, Benjamin R. tenOever, Adolfo Garcia-Sastre


We designed a barcoded viral library of recombinant influenza A viruses expressing a selection of NS1 from different strains, hosts, countries and years. Each virus was individually rescued and deep-sequenced using the Illumina MiSeq platform to verify viral genome integrity and sequence. This dataset includes Illumina deep-sequencing raw data of each virus included in the final library (barcodes 1-123).



Icahn School of Medicine at Mount Sinai Department of Microbiology


Virology, Illumina MiSeq