Searching for biomarkers candidates of autism spectrum disorder with metabolic disorders. Evidences for a possible role of proteins and amino acids content in urine.

Published: 8 May 2023| Version 3 | DOI: 10.17632/78ygyh8sb6.3


Autism spectrum disorder (ASD) is a developmental disorder characterized by conditions that involve areas of social interaction, communication and behavior as well as sensory sensitivity. At the beginning of the eighties researchs indicated the ASD of association with changes in protein metabolism and amino acids. To identify protein and amino acid profile in the urine of children with ASD and compare with a control group of urine samples. Prospective cross-sectional study, with a case-control design. The cases were children (n = 22) with ASD, male in the age group of 3 to 10 years who were compared with children (n = 22) with typical development, matched by sex and age. The quantification of total proteins was performed using the Bradford method and determination of the amount and amino acid composition by ultra-efficient liquid chromatography (CLUE). The results showed changes in the concentration of protein and amino acids arginine, glycine, leucine, threonine, aspartic acid, alanine, histidine, tyrosine in the urine of children with ASD. The total protein concentration and amino acid profile in urine are good candidates for biomarkers for individuals with ASD, but for this to be concrete, there is a need to develop studies with a larger number of participants.


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Instituto Butantan


Biomarkers, Protein, Autism Spectrum Disorder, Urinary Analysis