The inhibitory rates on the SGC-7901 cells treated with TSN or 5-FU for different time

Published: 16 November 2021| Version 1 | DOI: 10.17632/7brc5k98p7.1
Contributor:
xuepeng fu

Description

Supplementary data 1. The SGC-7901 cells were seeded on plates filled with RPMI1640 medium at a density of 1×104 cells/well in 96-well plates, and cultivated for 24 h to make them adhere on plates, and then treated with TSN and 5-fluorouracil (5-FU) respectively to reach a final concentration of 0, 20, 40, 60, 80, 100 nmol/L for 24, 48 and 72 h. Subsequently,the cells were incubated with 0.5μg/μL 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl- tetrazolium bromide (MTT) at 37℃ for 4h. The absorbance was measured using a Multi-well Plate Reader (Bio-Rad, Hercules, California, USA) at the wavelength of 570 nm. Cell inhibitory rate was calculated according to the formula: (1-absorbance of the cells treated with drug/absorbance of untreated control cells) ×100%. Supplementary data 2. The SGC-7901 were grown on Histogrip coated glass coverslips (Invitrogen, Carlsbad, CA, USA) in plates at a density of 1×104 cells/60-mm dish, and separately treated with TSN (0, 50, 70, 90 nmol/L) and 5-FU (70 nmol/L) for 48 h. Afterwards, the medium was discarded, 1 mL phosphate buffer solution (PBS) and 200 µL acridine orange solution (0.5 μg/μL) were added to the wells of the plates, respectively, then the plates were kept at room temperature for 3~5 min. The cellular morphologies were observed, and photographed using a laser confocal microscopy (Leica TCS SP8, Germany).

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The SGC-7901 cells were seeded on plates filled with RPMI1640 medium at a density of 1×104 cells/well in 96-well plates, and cultivated for 24 h to make them adhere on plates, and then treated with TSN and 5-fluorouracil (5-FU) respectively to reach a final concentration of 0, 20, 40, 60, 80, 100 nmol/L for 24, 48 and 72 h. Subsequently,the cells were incubated with 0.5μg/μL 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl- tetrazolium bromide (MTT) at 37℃ for 4h. The absorbance was measured using a Multi-well Plate Reader (Bio-Rad, Hercules, California, USA) at the wavelength of 570 nm. Cell inhibitory rate was calculated according to the formula: (1-absorbance of the cells treated with drug/absorbance of untreated control cells) ×100%. The SGC-7901 were grown on Histogrip coated glass coverslips (Invitrogen, Carlsbad, CA, USA) in plates at a density of 1×104 cells/60-mm dish, and separately treated with TSN (0, 50, 70, 90 nmol/L) and 5-FU (70 nmol/L) for 48 h. Afterwards, the medium was discarded, 1 mL phosphate buffer solution (PBS) and 200 µL acridine orange solution (0.5 μg/μL) were added to the wells of the plates, respectively, then the plates were kept at room temperature for 3~5 min. The cellular morphologies were observed, and photographed using a laser confocal microscopy (Leica TCS SP8, Germany).

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Qiqihar University

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Inhibitory Control Testing

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