Dataset for High-Dose Poultry Litter Biochar Application Significantly Alters Soil Bacterial Community

Published: 14 June 2024| Version 1 | DOI: 10.17632/7c5yxkm6s4.1
Avete Vieira Lima, Diogo Paes da Costa, Gustavo Pereira Duda, Jamilly Alves de Barros, José Romualdo de Sousa Lima1, Vanilson Pedro da Silva, Márcio Henrique Leal Lopes, Claude Hammecker, Ademir Sergio Ferreira Araujo, Erika Valente de Medeiros


Application of biochar from plant straw has been known to change the soil chemical properties, which contribute to driving the responses of soil microbiome. However, little is known about the effect of doses of biochar from poultry litter on soil bacterial community. In this study, we compared different doses of biochar derived from poultry litter on the composition, structure, diversity, and interactions of the bacterial community in a sandy soil. Six treatments were compared: a) control; b) lowest dose of biochar (Bio_10t = 10 t ha-1); c) highest dose of biochar (Bio_40t = 40 t ha-1); d) chicken manure (E.Av = 5 t ha-1); e) chemical fertilizers (Mineral = NPK fertilizer); and f) Poultry litter (C.Av = 10 t ha-1). Soil samples were collected and the V3-V4 variable region of the 16S rRNA gene was sequenced to assess the bacterial community. The highest dose of biochar changed the structure of the bacterial community clustering separately from the other treatments, being drove by higher organic C, P, and K content. In addition, the application of biochar decreased the richness and diversity of the bacterial community. The application of biochar changed the bacterial network decreasing the number of edges (1423 and 1849 edges with the doses of 10t and 40t, respectively) as compared to the control (3116 edges). In addition, the application of 40t biochar decreased the number of positive edges as compared to the application of 10t biochar. This study showed that the application of biochar obtained from a poultry litter in high dose significantly alters the composition, structure, diversity, and microbial interactions of bacterial community.



Microbiome, Agricultural Soil, Prokaryote