MiRNA and Target Gene Data of Combination Treatment In Biliary Atresia Rat Liver: Preconditioned Extracellular Vesicles Derived from Umbilical Cord Mesenchymal Stem Cells and Hepatocyte Growth Factor

Description

This dataset encompasses miRNA and target gene expression profiles obtained from preconditioned extracellular vesicles (EVs) derived from umbilical cord mesenchymal stem cells (UC-MSCs) treated with hepatocyte growth factor (HGF) in a biliary atresia (BA) rat liver model. Biliary atresia is a severe neonatal liver disease characterized by inflammation and obliteration of bile ducts, leading to liver cirrhosis. The dataset includes detailed miRNA sequencing data and gene expression analysis from rat liver tissues treated with these preconditioned EVs. This study aims to explore the therapeutic potential and underlying molecular mechanisms of HGF-preconditioned EVs from UC-MSCs in ameliorating liver damage in BA. The data is crucial for understanding the regulatory roles of miRNAs in liver regeneration and the specific target genes involved in this process.

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Methods: 1. Isolation and Preconditioning of UC-MSCs: - UC-MSCs are isolated from human umbilical cord tissue and cultured under standard conditions. - Preconditioning involves exposing UC-MSCs to specific stimuli to enhance the secretion of therapeutic EVs. 2. Extraction of EVs: EVs are harvested from the conditioned medium of preconditioned UC-MSCs using ultracentrifugation techniques. 3. Characterization of miRNA Profiles: Nanostring and bioinformatics analysis are employed to identify and quantify the miRNAs present in the EVs. 4. Identification of Target Genes: In silico analysis is used to predict the target genes of the identified miRNAs, focusing on those involved in liver regeneration and fibrosis reduction.

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