Anti-Inflammatory Potential of Goldenberry-Derived Exosome-Like Nanoparticles (GDEN) in Macrophage Polarization

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Abstract under review: Overpopulated M1 macrophages can trigger chronic inflammation. Plant-derived exosome-like nanoparticles have been reported to show beneficial bioactivities. Aim To isolate PDEN from goldenberry fruits and evaluate its anti-inflammatory potential in macrophage polarization. Methods GDEN were isolated by centrifugation and precipitation methods. LPS-induced RAW 264.7 cells were treated with GDEN before being evaluated with NO production assay and flow cytometry of CD80 and CD209. Results GDEN averaged 227,7 nm in size and spherical shape. GDEN 40 µg/mL decreased NO production in LPS-induced cells. Flow cytometry showed that CD209 (M2 marker) positive cells were up-regulated after being treated with 20 µg/mL GDEN. Conclusion GDEN showed anti-inflammatory potential through the ability to reduce M1 macrophages product and promote M2 polarization. Files consist of: 1. BCA assay: Raw data and standard curve for BCA assays in determining GDEN concentration. 2. MTT assay: Raw data and analysis for cell viability after being treated with GDEN or LPS. 3. NO assay for LPS only: Raw data and analysis of LPS-induced cells NO production. Data consists of standard curve, absorbance to NO concentration conversion, and approximate calculation for NO production per cell. 4. NO assay for LPS+GDEN: Raw data and analysis of LPS-induced cell NO production after being treated with GDEN in various concentrations. Data consists of standard curve, absorbance to NO concentration conversion, and approximate calculation for NO production per cell. 5. FC CD80 and CD209: Raw data and analysis of CD80 and CD209 flow cytometry results.

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