GO Enrichment Analysis, KEGG Pathway analysis, and STRING analysis of PFAS associated proteins
This research sought to identify more possible substrates that PFAS can deamidate by mass spectrometry. We purified PFAS associated proteins using anti-FLAG M2 magnetic beads and identified 441 proteins. We analyzed the data set using bioinformatic tools including GO annotation, KEGG pathway analysis, and STRING analysis. We confirm predicted protein interaction of PFAS with four proteins by co-immunoprecipitation and western blotting.