Data on volatile secondary lipid oxidation products in potato fruit juice from five industrial potato cultivars

Description

This study aimed to quantify eight volatile secondary lipid oxidation products in the fruit juice of five industrial potato cultivars and to assess whether significant differences exist among them. Of the eight target compounds, five were successfully detected. The data file is organized into three sections. In section 1 (yellow 1)), the five potato cultivars are listed along with the measured concentrations of detected volatiles (ng/0.5 mL potato fruit juice, PFJ). Data are based on three biological replicates (R1, R2, and R3), and the mean and standard deviation (SD) have been calculated for each compound. Section 2 (yellow 2)) presents the mean concentrations of the detected volatiles expressed as ng/mL PFJ, while section 3 (yellow 3)) shows the corresponding standard deviations. The results indicate significant differences among potato cultivars in the levels of volatile oxidation products. Notably, 2-pentylfuran and 2-nonenal are present at relatively high concentrations in PFJ, whereas the other detected compounds occur at lower levels. This pattern provides insight into the predominant lipid oxidation pathways in potato. Further details on data acquisition are provided in the “Steps to reproduce” section. Overall, the data demonstrate clear cultivar-dependent variation in the concentration of some key volatile lipid oxidation products.

Steps to reproduce

1 Preparation of Potato Fruit Juice PFJ from each variety was prepared in three batches. The potatoes were washed thoroughly to remove dirt and sand, then processed using a twin-screw Angel Juicer 8500S (Angel® Juicer, South Korea), which produced juice and pulp. The juice was centrifuged at 4700 x g for 10 min at 4 °C in a Multifuge 3SR (Fisher Scientific™, Germany) to remove starch and insoluble plant fibers. The resulting PFJ was immediately frozen using liquid nitrogen and stored at -21 °C until further analysis. 2 Identification and Quantification of Volatile Compounds To quantify volatile off-flavor compounds in PFJ (Section 1), 0.5 mL of each sample was mixed with 1.5 mL of MilliQ-water. Each sample received 10 μL of internal standard stock, providing 100 ng of Hexanal-d12, Benzaldehyde-d6, 2-Pentylfuran-d11, and Nonanal-d18. Volatile secondary lipid oxidation products in the samples were then identified and quantified using a head-space solid phase microextraction gas chromatography mass spectrometry (HS-SPME GC-MS) system from Agilent Technologies (Santa Clara, California, USA), based on the method described by Dalsgaard et al. (2010) with minor modifications. The samples were placed in a CTC Combi Pal autosampler, heated to 50 °C, and agitated at 500 rpm. Volatile compounds were collected from the headspace of samples using an SPME Fiber 57343-U (75 µm Car/PDMS) (Scientific Laboratory Supplies Ltd, Nottingham, England) over a 30 min period. These compounds were then desorbed onto an Agilent 7890A Gas Chromatograph equipped with an HP5-MS column (30 m length, 0.25 mm inner diameter, 0.25 μm film thickness, Agilent Technologies, California, USA). Helium was used as the carrier gas at a flow rate of 1 mL/min. The column temperature was initially set at 35 °C and increased at a rate of 4 °C/min until reaching 120 °C, after which it was further raised at 40 °C/min to a final temperature of 250 °C. An Agilent 5975 Mass Selective Detector was used for detection, operating in selected ion monitoring (SIM) mode. The quadrupole temperature was set to 150 °C with a fragmentation voltage of 70 eV, while the ion source and interface temperatures were maintained at 230 °C and 280 °C, respectively. Detection focused on specific ions corresponding to hexanal, hexanol, heptanal, benzaldehyde, 2-pentylfuran, 2,4-heptadienal, nonanal, and 2-nonenal. The concentrations of these compounds were then determined using an external standard curve (5-1000 ng) from the same tray, with internal standards Hexanal-d12, Benzaldehyde-d6, 2-Pentylfuran-d11, and Nonanal-d18 included to ensure accurate quantification. Hexanal-d12 was used as the internal standard for hexanal, hexanol, and heptanal, while Nonanal-d18 served as the internal standard for nonanal, 2-nonenal, and 2,4-heptadienal. Benzaldehyde-d6 and 2-Pentylfuran-d11 were only used as the internal standards for benzaldehyde and 2-pentylfuran, respectively.

Institutions

• Aarhus University

  Central Jutland, Aarhus

Categories

Funders

• REFINES project supported by Plant2Food

  Grant ID: NNF22SA0081019

Licence