Metabolomic profile of H1N1-infected human lung epithelial A549 cell after mannose treatment.
Description
To comprehensively understand the metabolic alterations induced by mannose treatment, we conducted a targeted metabolomics analysis with a specific emphasis on glucose homeostasis. Our study focused on human lung epithelial A549 cells infected with H1N1 virus. Intriguingly, we observed a significant increase in the levels of 3-phosphoglycerate and lactic acid following H1N1 infection, indicating perturbations in glycolysis. However, these metabolic changes were effectively reversed upon mannose treatment. Additionally, we investigated the impact of mannose treatment on the tricarboxylic acid (TCA) cycle, which is downstream of glycolysis. Our results revealed a similar trend of metabolic alterations in the TCA cycle, suggesting that mannose treatment not only influences the pools of glycolytic intermediates but also affects the metabolites associated with the TCA cycle. These findings shed light on the broader impact of mannose treatment on glucose metabolism and provide valuable insights into the potential therapeutic implications for combating viral infections. To investigate the effects of mannose treatment on glycolysis and the tricarboxylic acid (TCA) cycle, we conducted a series of experiments using A549 cells. The cells were pre-treated with either mannose (25mM) or PBS overnight. Subsequently, the cells were infected with H1N1 virus at a multiplicity of infection (MOI) of 5 for an additional 24 hours. After the treatment period, targeted metabolomics analysis of glycolysis and the TCA cycle was performed. The obtained results were normalized based on cell number. Statistical analysis was conducted using one-way ANOVA with Dunnett's post-hoc test, with multiple comparisons against the H1N1-PBS group. Each treatment was repeated four times (n=4) to ensure robustness and reliability of the findings. These experiments provide valuable insights into the impact of mannose treatment on the metabolic pathways associated with viral infection, offering potential avenues for therapeutic interventions.