Data for: Expression of Bacillus licheniformis chitin deacetylase in E. coli pLysS: Sustainable production, purification and characterisation

Published: 28-03-2019| Version 1 | DOI: 10.17632/7n4txwmsd4.1
Contributor:
keyur raval

Description

Supplementary figure 1 shows the effect of different types of media on CDA activity. The cultures grown in TSB medium exhibited the maximum CDA activity as compared to the other investigated media. Supplementary figure 2A and 2B demonstrate the effect of the different nitrogen sources and different glycerol concentrations on the CDA expression. Yeast extract and beef extract exhibited almost similar enzyme expression of about 160 U/ml, followed by peptone and tryptone. Supplementary figure 3 demonstrates the SDS-PAGE picture of the various stages of purification. The purified protein showed the size of about 29 kDa, which is similar to the expected size.

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