Dataset_LK

Published: 3 June 2024| Version 1 | DOI: 10.17632/7njhhgwtz9.1
Contributor:
Laura Köcher

Description

This dataset includes MATLAB code and raw data. Raw data (as .xlsx files) contains behavioral tracking obtained ZebraLab tracking software for each timepoint and each condition (controls, low- and high-impact injury). Controls were measured simultaneously with the respective injury group (low-impact injury condition within the folder 'curve01' and high-impact injury condition within the folder 'curve02').

Files

Steps to reproduce

(1) readout.m extracts oscilloscope readout saved as excel file from experimental apparatus and plots motion trajectories. (2.1.) DistTrav_extract_AcousticResp.m extracts data used to analyze escape responses from excel file and saves individual recordings as array. (2.2.) DistTrav_extract_SpontActivity.m extracts data used to analyze spontaneous activity from excel file and saves individual recordings as array. (3) array_singleRep.m combines arrays from all six individual timepoints (2) as array for one biological replicate. (4) FormatAllReps.m combines arrays from all three biological replicate (3) for both conditions (low- and high-impact injury) as array. (5) AnalysisAllReplicates.m: Final analysis and plots. - calculates mean escape responses for all larvae of each replicate (for SD) and overall mean (mean) - single exponential fitting during the habituation phase of the behavioral assay of the escape responses for each individual larvae - calculates Baseline responsiveness and Acoustic Sensitivity for each single larvae - calculates spontaneous activity (defined as movements with speed <20mm/s) (6) Statistics.R: Statistical analyses of the data extracted from the behavioral tracking performed using RStudio

Institutions

Universitat Zurich, UniversitatsSpital Zurich

Categories

Neuroscience, Behavioral Neuroscience, Neurology

Licence