Raw and processed FTIR, XRD, TGA and protein diffusion Data of Microcrystalline Cellulose film from Mangosteen Rind for Potential Membrane Applications

Description

This dataset contains raw and processed characterization data obtained from Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA) of cellulose-based films prepared from mangosteen rind. The dataset is intended to support the structural, chemical and thermal characterization of the prepared material and to facilitate reproducibility and further analysis. Further data sets for calculating protein diffusion across the membrane are also included.

Steps to reproduce

1. FTIR analysis (GraphPad Prism): Import the FTIR spectral data recorded using a JASCO FT/IR-4600 spectrophotometer over 550–4000 cm⁻¹. Plot the wavenumber (cm⁻¹) against transmittance (%) as an XY line graph. Format the axes and legends and export the spectra for comparison of characteristic absorption bands in MCC and MCC films. 2. XRD Analysis (OriginPro): Import the XRD data collected using an Empyrean Series III diffractometer with Cu-Kα radiation (λ = 1.54 Å), assigning 2θ (°) as X and intensity as Y. Plot the diffraction pattern, use Peak Analyzer for baseline correction and peak fitting. Integrate the crystalline and amorphous contributions using a consistent fitting model and calculate the crystallinity index as: CI (%)=(Crystalline peak area)/(Peak area of both crystalline and amorphous region)×100 Save the OriginPro project with the baseline, fitting, and integration settings. 3. TGA Analysis (GraphPad Prism): Import the TGA data obtained using a PerkinElmer TGA 4000 under nitrogen at a heating rate of 20 °C/min. Plot the temperature (°C) against the mass remaining (%) as an XY line graph. Format and export the thermogravimetric profile to illustrate the thermal degradation behaviour of the MCC film. 4. Protein Diffusion Analysis (Excel and GraphPad Prism): Use the BSA calibration data to establish the absorbance–concentration relationship. Calculate protein concentrations from the Lowry assay measurements and determine cumulative BSA release at each 5-minute interval over 30 minutes, accounting for previously withdrawn aliquots where applicable. Organize replicate measurements in Excel, calculate mean and standard deviation where appropriate, and plot cumulative protein release against time in GraphPad Prism. Data Availability: Raw numerical data, processed datasets, OriginPro XRD analysis project, exported FTIR and TGA graphs, and an Excel workbook containing the BSA calibration and protein-release calculations are provided for independent verification and reproducibility.

Institutions

• Easwari Engineering College

  Tamil Nadu, Chennai

Categories

Licence