Cell Reports; Nychyk et al 2021; Figure 7; Protein quality and quantity influence the effect of dietary fat on weight gain and tissue partitioning via host-microbiota changes

Published: 11-05-2021| Version 1 | DOI: 10.17632/7rb9x9xcw3.1
Kanishka Nilaweera,
Oleksandr Nychyk,
Agata Rudolf,
Yolanda Piotrowicz,
John Speakman


This experiment was to done to analyze the impact of dietary macronutrients and the associated microbiome on body weight. We used 2 different diets and one mouse strain (C57BL/6). This file contains the individual data of male C57BL/6 mice exposed to 12 weeks feeding on one of 2 different diets. Body weight recorded every 3-4 days for each mouse; final body weight gain calculated at the end of each treatment period. 9 to 10 animals were exposed to each diet. Dietary treatments consisted of one level of dietary fat (55% by energy) combined with one level of protein (30% by energy) with either casein (CAS) or whey protein isolate (WPI). Full details of the dietary compositions can be found in the supplementary table 1. First, animals were fed for 4 weeks with either 30% CAS or WPI in a 55% fat diet and switched to a 4-week antibiotic (ABX) treatment (i.e., CAS-ABX, WPI-ABX) or they continued to receive the same diet (CAS-control, WPI-control). For the final 4 weeks of the experiment, the two control groups acted as faecal donors for the two groups which had received antibiotics. Complete meta-data are in the meta-data tab of the file. Means for each diet derived from these individual values are presented in Figure 7B and C.