Supplementary figures and data for temperature effects on pollen tube progression and associated physiological and metabolic profiles in ‘Harlikar’ apple
Description
This dataset contains supplementary figures (Figures S1–S5) and supplementary tables (Tables S1–S10) accompanying the study “Temperature effects on pollen tube progression and associated physiological and metabolic profiles in ‘Harlikar’ apple”. The study examined ‘Harlikar’ apple flowers hand-pollinated with ‘Fuji’ pollen under temperature treatments of 15, 20 and 25 °C. The supplementary figures present the experimental workflow, ethylene-release time courses, an extended heatmap of volatile features, and OPLS-DA score plots and permutation tests. The supplementary tables provide quantitative measurements, processed data and statistical results for reactive oxygen species-associated staining, ethylene release, endogenous hormones, volatile metabolites, and associations between hormone and volatile profiles. Supplementary Figures contains Figures S1–S5 and their legends. Supplementary Tables contains Tables S1–S10, a contents worksheet and an ethylene calculation-parameters worksheet. UIS denotes the unpollinated initial stage, OES denotes the first observed ovary-entry stage of pollen tubes, and HAP denotes hours after pollination. OES samples were collected at 72 HAP at 15 °C and at 48 HAP at 20 and 25 °C.