Original paired-end transcriptome outputs from the RNAseq analyses of the false black coral Savalia savaglia
Description
This dataset contains outputs generated from the original paired-end transcriptomic analyses of the false black coral Savalia savaglia. The dataset includes the following files: • 75282_ID2093_3-SAS_S416_L004_R1_001.fastq.P.qtrim.zip: Preprocessed forward reads used for the novo assembly, obtained from the paired-end RNA-Seq data of Savalia savaglia, containing high-quality sequences that passed quality trimming and filtering using Trimmomatic. • 75282_ID2093_3-SAS_S416_L004_R2_001.fastq.P.qtrim.zip: Preprocessed reverse reads used for the novo assembly, obtained from the paired-end RNA-Seq data of Savalia savaglia, containing high-quality sequences that passed quality trimming and filtering using Trimmomatic. • Assembly_Ss_PE.Trinity.fasta: Original, non-filtered de novo paired-end transcriptome assembly of Savalia savaglia, generated from 68 million PE reads using the Trinity Assembler. • Assembly_Ss_PE_Trinity.fasta_stats.txt: Statistical summary of the paired-end transcriptome assembly of Savalia savaglia. • Assembly_Ss_PE_Trinity.fasta.gene_trans_map: Transcript-to-gene mapping file generated during the paired-end transcriptome assembly of Savalia savaglia. • quant_Ss_PE.sf: Salmon output containing expression values for the assembled transcripts from the paired-end assembly of Savalia savaglia. Additionally, the dataset includes the following files from DIAMOND BLASTx analyses, which used the original de novo paired-end transcriptome assembly of the false black coral Savalia savaglia: • UniRef90_PE.diamond.blastx.outfmt6: BLASTx output file against the UniRef90 database, reporting the top alignment for each query (assembled transcripts) from the paired-end assembly of Savalia savaglia. • UniRef90_PE.diamond.blastx.outfmt6.grouped: Grouped BLASTx hits from the paired-end assembly of Savalia savaglia, designed to improve sequence coverage by combining multiple high-scoring segment pairs (HSPs). • UniRef90_PE.diamond.blastx.outfmt6.hist: Histogram summarizing the distribution of BLASTx hit lengths obtained from the paired-end assembly of Savalia savaglia. • UniRef90_PE.diamond.blastx.outfmt6.w_pct_hit_length: File providing percentages of hit lengths from BLASTx analyses of the paired-end assembly of Savalia savaglia, including the top hit's length and the percent of the length covered in the alignment.
Files
Steps to reproduce
The paired-end transcriptome of the false coral Savalia savaglia (Assembly_Ss_PE.Trinity.fasta) was assembled with Trinity v2.15.1 using a single kmer in forward mode. The associated outputs files were generated as part of the Trinity pipeline (https://github.com/trinityrnaseq/trinityrnaseq/wiki). Trimming of the raw reads was performed with Trimmomatic-0.39 (https://github.com/usadellab/Trimmomatic/releases/tag/v0.39).
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Funding
This work was supported by Centro Ricerche ed Infrastrutture Marine Avanzate in Calabria (CRIMAC) - Fondo FSC 2014-2020 - Piano Stralcio «Ricerca e Innovazione 2015-2017» – Programma Nazionale Infrastrutture di Ricerca (PNIR), CUP C64I20000320001.