EVALUATION OF THE EFFECT OF MATRICARIA RECUTITA AND PHOTODYNAMIC THERAPY ON TISSUE REPAIR IN ORAL MUCOSA OF RATS

Published: 21 April 2023| Version 1 | DOI: 10.17632/7txy2vskmw.1
Contributors:
Juliana Borges de Lima Dantas, Tila Fortuna,
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Description

Ulcers are common oral cavity lesions. The search for treatments that accelerate the healing of these lesions is of constant interest. Matricaria recutita is a plant with antimicrobial, anti-inflammatory, and healing properties, and antimicrobial Photodynamic Therapy (aPDT) eradicates microorganisms, which favors tissue repair. The objective of this study was to evaluate the effect of the topical use of chamomile with or without aPDT on tissue repair in the dorsum of the tongue of rats. Seventy-five male Wistar rats underwent standardized ulceration on the dorsum of the tongue using a punch of 5 mm diameter and were randomly allocated into the following groups: control (G1), Chamomile Fluid Extract (G2), Chamomile Infusion (G3), aPDT (G4), and Chamomile Infusion + aPDT (G5). On the 3rd, 7th, and 14th days postoperatively, euthanasia was performed, and the ulcers were measured using calipers. The presence of edema, inflammatory infiltrate, cellularity, re-epithelialization, and characterization of total collagen were evaluated using sections stained with Hematoxylin and Eosin and Red Sirius. Histomorphometric analyses of the percentage of total collagen, the distance from the basal layer to the epithelial surface, and the thickness of the stratum corneum were performed. G2 positively modulated the exudative and proliferative phases of repair, both clinically (p<0.0001) and histologically, whether in descriptive or inferential analyses (p<0.05). G3 showed a significant difference in clinical parameters compared with G1 (p<0.0001). G4 and G5 did not positively modulate tissue repair. The Chamomile Fluid Extract showed better outcomes for tissue repair in the rat tongue. There were differences in the clinical, histological, and histomorphometric patterns among this study’s experimental groups.

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Institutions

Universidade Federal da Bahia

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Animal Histology

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