PXR activation in 3T3L1 and NIH3T3 fibroblasts

Description

These data demonstrate the effects of pregnenolone 16a-carbonitrile, a pregnane x receptor agonist, in two murine fibroblast models on fibroblast function including in vitro scratch wound healing, cellular migration, proliferation, myofibroblast differentiation, and extracellular matrix production. There data are in support of a recently submitted manuscript.

Steps to reproduce

Cells were treated for 24 to 48 hours with vehicle (DMSO 0.025%) or PCN (20 uM) then effects on in vitro scratch closure, cellular migration by Boyden chamber migration assay, proliferation by MTT assay, TGF-b1 induced myofibroblast differentiation as indicated by aSMA, Col1a1, and Col3a1 expression was assessed. Please contact the authors for specific protocols, instruments, and reagents used.

Institutions

• Mississippi State University College of Veterinary Medicine

  MS, Mississippi State
• Mississippi State University

  MS, Mississippi State

Categories

Funders

• National Institute of Environmental Health Sciences

  North Carolina, United States

  Grant ID: 1R15ES035527-01

Licence