Data for: Numerical and length densities of microvessels in the human brain: Basic mapping of differences between the cerebral cortex, subcortical grey matter and white matter, pons and cerebellum

Published: 9 November 2017| Version 2 | DOI: 10.17632/7x9vs99564.2
Zbynek Tonar


Supplement 2. Volumes of fresh tissue samples, formalin-fixed blocks, and exhaustively cut paraffin-embedded serial sections. Analysis of the shrinkage of 30 tissue blocks is provided. The X-, Y-, and Z- dimensions of each block were measured immediately after dissection (fresh samples) and after formalin fixation using a digital caliper. The dimensions of the exhaustively cut block were measured using a calibrated microscope eyepiece (X- and Y-dimensions) and by multiplying the thickness of the histological sections by the number of sections per block (Z-dimension). Orientation of the X-, Y-, and Z- axes (i.e., length, width, and height) of each block was preserved carefully during all processing.



Histology, Neuroanatomy, Stereology