Protein ID-Mass spec_OCT4 interacting protein in neuroblastoma cells

Published: 1 February 2018| Version 1 | DOI: 10.17632/7y95vvpw9g.1
Min Kang


OCT4-mycDDK recombinant protein was expressed in a human neuroblastoma cell line. OCT4-mycDDK protein was pulled down by immunoprecipitation (IP) using EZview Red anti-FLAG M2 affinity purification, and the protein was separated on SDS-PAGE to cut the appropriate bands out to be analyzed at the Proteomics Core Facility of University of Texas Southwestern Medical Center, Dallas, TX