Detoxification of aflatoxin B1 by essential oils of medicinal herbs

Published: 4 October 2021| Version 2 | DOI: 10.17632/86g3w9vhbn.2
Velazhahan Rethinasamy,


The essential oils (EO) extracted from the medicinal plants viz., Heliotropium bacciferum, Ocimum dhofarense and Zataria multiflora were diluted with methanol (1:10, v/v) and 250 μl of EO was mixed with AFB1 (50 μg/l) in a microcentrifuge. Following a 24-h incubation at 25°C, AFB1 in the mixture was extracted with 250 μl of chloroform. The chloroform fraction was evaporated at 60°C using a water bath and the residue was quantified by enzyme-linked immunosorbent assay using a commercial kit (RIDASCREEN Aflatoxin B1; R-Biopharm AG, Darmstadt, Germany).



Detoxification, Mycotoxin, Medicinal and Aromatic Plants