Genome-wide characterization of the 14-3-3 gene family of Lycium barbarum L. and its expression pattern analysis under Fusarium root rot infection

Published: 7 November 2024| Version 1 | DOI: 10.17632/87rbss8vyj.1
Contributor:
kun zhao

Description

The 14-3-3 proteins are crucial for plant growth, development, and stress response. Lycium barbarum L., a major cash crop and medicinal plant, offers significant economic and ecological benefits. Nineteen genes encoding proteins with PF00244 typical conserved structural domains were found by looking through the whole genome of the L. barbarum. Phylogenetic analysis of L. barbarum and other plants showed that 14-3-3 proteins can be classified into ε and non-ε categories; gene structure analysis, gene length, and number of introns varied greatly among members of the 14-3-3 gene family of L. barbarum. Members of the LbGRFs family are unevenly distributed on chromosomes. The investigation of promoter cis-acting elements revealed that the LbGRFs promoter has cis-acting elements for several hormones and stressors. An analysis of covariance within the species indicates that fragment duplication is the primary cause of the 14-3-3 gene family amplification in L. barbarum. Several 14-3-3 genes experienced evolved from a common ancestor, according to the results of the interspecies covariance analysis of the tomato, Arabidopsis thaliana, and L. barbarum. In this work, we completed the genome-wide identification, evolution, and expression investigation of the L. barbarum's 14-3-3 gene family. These results provide important information for further study of the function of 14-3-3 gene family in L. barbarum. The results of qRT-PCR showed that the expression level of 14-3-3 gene family in L. barbarum was affected by root rot bacteria, which may play an important role in the resistance of L. barbarum to root rot disease.

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Institutions

Ningxia University, Nanjing Forestry University

Categories

Molecular Biology, Bioinformatics, Plant Biology

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