Airway Epithelial Transcriptome in Lung Transplant Recipients with Cystic Fibrosis and/or Pseudomonas

Published: 09-07-2020| Version 1 | DOI: 10.17632/89nzfyvkvc.1
Contributors:
Daniel Dugger,
Monica Fung,
Chaz Langelier,
Saharai Caldera,
Steven Hays,
Jon Singer,
Lorianna Leard,
Jeff Golden,
Rupal Shah,
Marry Ellen Kleinhenz,
John Greenland

Description

Lung transplant recipients with cystic fibrosis were found to tolerate Pseudomonas aeruginosa infection better than non-CF individuals. We hypothesized that differences in airway immune responses might relate to these more favorable outcomes. We prospectively collected airway brushes from lung transplant recipients with PsA+ cultures from 9 CF and 6 non-CF individuals. These were matched 1:1 with PsA- cases, and we performed next-generation RNA sequencing to evaluate the host transcriptome. RNA was sequenced using Illumina-based next generation sequencing and count data are supplied as a GEO-format count matrix. We also cultured airway epithelial cells and performed differential DNA methylation analysis using the Infinium HumanMethylation450 BeadChip. The data are included here as in EPIC report format.

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