Masters Thesis - Phenolics in Australian Grain Crops
Description
The NIR and MIR datasets contain sample information, chemical reference data and the mean NIR/MIR spectra for each sample. The x-axis variables across the top of the NIR datasets are provided in nm; MIR data in cm-1. CUPRAC, FRAP, TP and TMA values are given as mg/100 g. Moisture and protein are in % w/w. Starch, amylose, amylopectin, vicine, convicine and total VC are in mg/g. The phenolic_contents datasets show the results from HPLC analysis of individual phenolic compounds present in each sample. The content of individual phenolic acids are given in mg/kg. All measurements are expressed on an oven-dried weight basis. Abbreviations used: HKW = hundred kernel weight CUPRAC = cupric reducing antioxidant capacity FRAP = ferric reducing antioxidant potential TP = total phenolic content TMA = total monomeric anthocyanin content Total_VC = sum of vicine and convicine contents
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Steps to reproduce
Chemical analysis was conducted following protocols previously published by our laboratory (DOI: 10.1002/leg3.28). Near-infrared spectra were collected from the flour samples using an Antaris II FT-NIR Analyzer (Thermo Scientific; USA). The instrument was operated in reflectance mode, using the integrating sphere with a rotating sample cup (30 mm diameter). Spectra were collected between 1000-2500 nm (10,000-4,000 cm-1), as the mean of 32 scans (resolution of 8 cm-1). Spectra were collected in triplicate, repacking the sample cup with fresh flour each time. Spectra were exported in *.csv format, with the mean of the triplicate spectra for each sample used in subsequent analysis. Mid-infrared spectra were collected from the flour samples using a Bruker Alpha FTIR (Fourier transform infrared) spectrophotometer (Bruker Optics Gmbh, Ettlingen, Germany) fitted with a platinum diamond ATR single reflection module. Air was used as a reference background; the background measurement was performed every 10 samples. MIR spectra were recorded between 4000 and 400 cm-1 using the OPUS software version 7.5 (Bruker Optics Gmbh, Ettlingen, Germany) as the average of 24 scans at a resolution of 4 cm-1. Replicate spectra were collected from each sample (3 for mungbean and chickpea and 5 for faba bean and wheat), repacking the instrument with fresh flour each time. The mean of these replicate scans were used in subsequent analysis.