RNAseq results using kidney cortex from mice receiving human immunoglobulin light chains
Description
We hypothesized that immunoglobulin free light chains (FLC) generated oxidative stress in the kidney and promoted significant changes in RNA expression when compared to vehicle treated control mice. Analyses were performed using kidney cortex obtained from mice that received human FLC or vehicle (PBS) control parenterally for 10 days. There are four animals in each group. Total RNA was isolated from right kidney cortex and RNA was sequenced on NextSeq500 System and the library was prepared with the Agilent SureSelect Stranded mRNA kit.
Files
Steps to reproduce
Total RNA was isolated from right kidney cortex in standard fashion. RNA was sequenced on a NextSeq500 System and the library was prepared with the Agilent SureSelect Stranded mRNA kit. Raw sequencing (fastq) files were subjected to quality control analysis using FastQC (v0.11.5) [http://www.bioinformatics.babraham.ac.uk/projects/fastqc/]. Cleaned sequence reads were aligned to mouse genome (GRCm38) using TopHat v2.1.0 [https://www.ncbi.nlm.nih.gov/pubmed/23618408] or STAR v2.5.3.a [https://www.ncbi.nlm.nih.gov/pubmed/23104886]. Differential expression analysis was performed using DESeq2, following standard protocol [https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html].