single-cell circadian rhythm in live zebrafish larva

Published: 11-12-2017| Version 1 | DOI: 10.17632/8ddwf35d43.1
Contributor:
Haifang Wang

Description

The files (in tiff format) are 3D reconstructions at different time points for each individual fish, aligned using CMTK toolkits except for Supplementary Movie 3. Supplementary Movie 1&2. The combined image stacks of the whole brain using two-photon imaging . The fish was imaged from 3.5 dpf to 7.5 dpf every 12 hours (9 stacks). The fish was raised under LD condition. Supplementary Movie 3. Confocal 3D reconstructions of zebrafish pineal gland. Zebrafish larvae was co-labeled with nr1d1:VNP (blue) and aanat2:mRFP (red) . Supplementary Movie 4-9. The combined image stacks of the pineal gland using two-photon imaging under LD condition (LD fish1-6). The fish was imaged from 3.5dpf to 6.5dpf every 12 hours (7 stacks). Supplementary Movie 10-13. The combined image stacks of the pineal gland using two-photon imaging under DD condition (DD fish 1-4). The fish was imaged from 3.5dpf to 6.5dpf every 12 hours (7 stacks). Supplementary Movie 14&15 (LD fish 7&8). The combined image stacks of the pineal gland using two-photon imaging under LD condition. The fish was imaged at 5.0 dpf in every two hours (12 stacks). Supplementary Movie 16-18 (DD fish 5-7). The combined image stacks of the pineal gland using two-photon imaging under DD condition. The fish was imaged at 5.0 dpf in every two hours (12 stacks).

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