Data of microRNAs and Target Gene in Preconditioned compared to Naive Extracellular Vesicles Derived from Umbilical Cord Mesenchymal Stem Cells
Description
This dataset contains information on the microRNAs and their target genes found in extracellular vesicles (EVs) derived from umbilical cord mesenchymal stem cells (UC-MSCs). The data compares microRNA profiles between preconditioned and naive (non-preconditioned) EVs. Data Applications: - Therapeutic Potential: Understanding how preconditioning alters microRNA content in EVs can help in designing better therapeutic strategies using UC-MSC-derived EVs. - Mechanistic Insights: Insights into how preconditioned EVs affect target gene expression can elucidate mechanisms of action in various physiological and pathological processes. - Biomarker Discovery: Identification of specific microRNAs and target genes can serve as biomarkers for monitoring the efficacy of preconditioning treatments. This dataset is crucial for researchers focusing on the therapeutic use of EVs, particularly in enhancing the regenerative potential of UC-MSCs through preconditioning strategies.
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Steps to reproduce
Methods: 1. Isolation and Preconditioning of UC-MSCs: - UC-MSCs are isolated from human umbilical cord tissue and cultured under standard conditions. - Preconditioning involves exposing UC-MSCs to specific stimuli to enhance the secretion of therapeutic EVs. 2. Extraction of EVs: EVs are harvested from the conditioned medium of preconditioned UC-MSCs using ultracentrifugation techniques. 3. Characterization of miRNA Profiles: Nanostring and bioinformatics analysis are employed to identify and quantify the miRNAs present in the EVs. 4. Identification of Target Genes: In silico analysis is used to predict the target genes of the identified miRNAs, focusing on those involved in liver regeneration and fibrosis reduction.