Data for: Improving Techniques to Study Equine Cervical Mucociliary Clearance

Published: 24-04-2019| Version 1 | DOI: 10.17632/8gyk38b7t2.1
Contributors:
Robert Causey,
Anna Richard,
Alec Toothaker,
Martin Stokes,
Chelsie Oldfield,
Melissa Hawkes

Description

Note: Dates displayed on video recordings (May 2016) do not match when the study was performed (July, August 2017). extendedview_appendedvideo.mp4 This is an extended video segment which includes all of the particles deposited on the cervix of the mare in vivo (1-5 um green microspheres, 70 um green microspheres, 50 um bichromal microspheres, and carbon). At 45 s into the video movement represented in Figure 5 can be observed. The particle is in the top right of the screen, just below the magnification marker. The particle moves abruptly to the right, but with a slight back ward (to the left) movement of the top surface of the microsphere. This movement was predicted prior to the study as evidence supportive of mucociliary propulsion from below, but in this video recording is too limited to constitute proof. Erythrocytes moving through capillaries can be seen, from 59 s, and with most clarity at 1 m in 20 s. Figure_4_appendedvideo Shows a flash of light from the particle circled in Figure 4. This is interpreted as the particle distorting the meniscus, possibly as a result of rotation. Figure_7_appendedvideo Fluid movement can be seen through small vessels in the upper left quadrant of the black box. Figure_8_appendedvideo Particles observed within the two boxes may be seen to be progressing independently. The green polyethylene microspheres (blue box in Figure 5) move convincingly to the lower right of the field. The carbon particles in the red box in Figure 5 are static or moving very slowly to the upper left. Note: Dates displayed on video recordings (May 2016) do not match when the study was performed (July, August 2017).

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