UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection. Alcántara et al., 2021.
Supplemental Data S1: Reaction recipe template, related to CRISPR/Cas12a-mediated detection section. Excel spreadsheet intended to be used for reagents and enzymes volume calculation for CRISPR/Cas reaction preparation. Data about the number of reactions, crRNA, and Cas12a concentrations must be replaced with the appropriate values. Recommended setup values for the fluorescence reader Synergy H1 (BioTek) are also indicated. Supplemental Data S2: Fluorescence ratio calculation template, related to Quantification and Statistical Analysis section. Excel spreadsheet intended to be used for fluorescence signal data analysis from the fluorescence reader Synergy H1 (BioTek) (or another similar alternative equipment). Fluorescence signal data must be replaced directly in the “Fluorescence analysis template” tab, whenever the experiment plate was set up according to the recommended plate layout for CRISPR/Cas reaction.
Steps to reproduce
For complete details on the use of these spreadsheets, please refer to "UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection" (Alcántara et al., 2021).