Recombinant O-GlcNAcylated human tau after GalNAzylation, biotinylation, trypsinization, coupling to streptavidin beads and β-elimination-Michael addition - RAW data files - nLC-Q Exactive HF acquisition
Published: 12 December 2022| Version 1 | DOI: 10.17632/8n5j45dnd8.1
Contributor:
Sebastiaan BijttebierDescription
Chemo-enzymatic derivatization (GalNAzylation, biotinylation) of O-GlcNAcylated recombinant human tau, trypsinization, enrichment via coupling to streptavidin beads, and release of tagged peptides by β-elimination – Michael addition reaction. nLC-HRMS (Q Exactive HF) analysis of final extract to monitor sulfited peptides for the localization of tau O-GlcNAcylation sites. Data were acquired in data dependent MS2 (ddMS2) mode and for low abundant O-GlcNAc peptides also in parallel reaction monitoring (PRM) mode.
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Categories
Post-Transcription Modification, Liquid Chromatography Mass Spectrometry, Tauopathy