Intraepithelial lymphocytes facilitate trypsin-independent PEDV infection and subsequent immune suppression via the CD69–STK17B axis
Description
The raw data of the study is available.
Files
Steps to reproduce
Figure2C The co-culture system was infected with the strong strain SX and the weak strain CV777 of PEDV. Cell precipitates and supernatants were collected at various time points post-infection, and viral copy numbers were quantified by substituting the data into the PEDV standard curve using RT-qPCR. Figure3C RT-PCR analysis of successive passages (F1-F5) of strain B18 in the co-culture system or Vero cells: 1-5 represent PIO-IEL co-culture + PEDV F1-F5; 6-10 represent Vero + Trypsin + PEDV F1-F5; 11-15 represent Vero + PEDV F1-F5; 16 indicates the negative control (NC), with the marker DL2000. Figure3D Detection of the viral copy number of the PEDV wild strain B18 in vitro during serial passages F1-F5 was conducted using RT-qPCR. Figure5A Gene expression profiles of lymphocyte cell surface receptors CD44, MADCAM-1, CD69, S1PR1, and STK17B in PIOs and IELs were assessed using RT-qPCR at 0-72 hpi, utilizing a co-culture system infected with the PEDV SX strain. Figure5B Western blot analysis was performed to detect protein expression levels of cell surface receptors at 72 hpi with PEDV. Figure5C The band signals of the target proteins were subjected to relative quantitative analysis using the gray value analysis method to determine the relative expression levels of the target proteins.
Institutions
- Northeast Agricultural UniversityHarbin, Harbin