The Effect Of Single Dose Albendazole (400 Mg) Treatment On The Human Gut Microbiome Of Hookworm-Infected Ghanaian Individuals

Published: 21 December 2022| Version 2 | DOI: 10.17632/8rrxrzrwpj.2
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Description

Microbes play an important role in human gut homeostasis, metabolic, immunologic and physiopathology of the body. A longitudinal study conducted during 2018-2021 in the Kintampo North Municipality in Ghana demonstrated low hookworm infection cure rates following treatment with a single dose of 400 mg albendazole in some communities. To investigate associations between hookworm infection and the gut microbiome, we examined faecal samples from consented participants who were either cured or remained infected after treatment. At each time point, fecal samples were collected prior to and 10-14 days after albendazole treatment of those who were infected. We used 16S rRNA amplicon sequencing of DNA extracted from stool samples to investigate the composition and biodiversity of the gut microbiota and to identify potential microbial biomarkers associated with treatment outcomes. This dataset contains bacterial 16s gene sequence reads with primers and barcodes pre-trimed from the amplification of the V3-V4 hypervariable region, sequenced on the NovaSeq 6000 Illumina platform following standard Illumina sequencing protocols. Sample Key: Exxx -Extraction Negative controls G1xxxx - Successful albendazole treatment G2xxxx - Failed albendazole treatment G3xxxx - Successive Reinfection after albendazole treatment Nxx - No Infection Controls

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Institutions

University of Illinois at Urbana-Champaign, University of Ghana Noguchi Memorial Institute for Medical Research, Yale University Department of Epidemiology of Microbial Diseases

Categories

Gut Microbiome

Funding

National Institutes of Health

U19AI129916

National Institute of Allergy and Infectious Diseases

R01AI132452

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