Mechanisms of readthrough mitigation reveal principles of GCN1 mediated translational quality control

Published: 16 May 2023| Version 2 | DOI: 10.17632/8rss6rghvb.2
Martin Mueller, Gopal Jayaraj, Ulrich Hartl


Readthrough into the 3′ untranslated region (3′UTR) of the mRNA results in the production of aberrant proteins. Metazoans efficiently clear readthrough proteins, but the underlying mechanisms remain unknown. Here, we show in C. elegans and mammalian cells that readthrough proteins are targeted by a coupled, two-level quality control pathway involving the BAG6 chaperone complex and the ribosome collision-sensing protein GCN1. Readthrough proteins with hydrophobic C-terminal extensions are recognized by SGTA-BAG6 and ubiquitylated by RNF126 for proteasomal degradation. Additionally, cotranslational mRNA decay initiated by GCN1 and CCR4/NOT limits the accumulation of readthrough products. Unexpectedly, selective ribosome profiling uncovered a general role of GCN1 in regulating translation dynamics when ribosomes collide at nonoptimal codons, enriched in 3′UTRs, transmembrane proteins and collagens. Dysfunction of GCN1 increasingly perturbs these protein classes during aging, resulting in mRNA and proteome imbalance. Our results define GCN1 as a key factor acting during translation in maintaining protein homeostasis.



Max-Planck-Institut fur Biochemie


Biochemistry, Cell Biology, Microscopy, Flow Cytometry, Western Blot