Imaging data 1 (3D-CIS) from "Interchromosomal contacts between regulatory regions trigger stable transgenerational epigenetic inheritance in Drosophila" by Fitz-James et al.
Published: 13 November 2024| Version 1 | DOI: 10.17632/8rz25rrtzj.1
Contributors:
Max Fitz-James, Description
Imaging data from Figures 5 and S11 of "Interchromosomal contacts between regulatory regions trigger stable transgenerational epigenetic inheritance in Drosophila" by Fitz-James et al.
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Drosophila embryos were collected and subjected to two-colour FISH, highlighting cytogenetic positions 37B and 89E. 3D stacks were collected from 3-5 different embryos. Optical sections were collected at 0.5 μm intervals along Z-axis using a Leica SP8-UV microscope or a Zeiss AXIO imager. Relative 3D distances between FISH signals were analyzed in approximately 80 to 120 nuclei per 3D stack using the Imaris software (Oxford Instruments).
Institutions
Institut de Genetique Humaine
Categories
Cell Biology, Microscopy, Drosophila