Large Drosophila Germline piRNA Clusters are Evolutionarily Labile and Dispensable for Transposon Regulation. Gebert et al. 2021

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Representative confocal projection of RNA-FISH signal for 20A sense probes (red) in w1118 and 20A(p)∆ piRNA cluster mutant Drosophila ovaries. DAPI (DNA, blue).

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RNA FISH was performed using Custom Stellaris® RNA FISH Probes, designed using the Stellaris® RNA FISH Probe Designer (Biosearch Technologies), as previously described (Trcek et al., 2015). RNA FISH Probes were labeled with Quasar670 to detect Cluster 2 (20A) sense mRNA sequence. FISH probes were made of 20-nt-long oligo pools, as listed in Table S5. Samples were mounted in Vectashield® media containing DAPI (Vector Laboratories). Fluorescent images were acquired with a Plan-Apochromat 40X/NA1.4 (oil immersion) objective on a Zeiss LSM 780 confocal microscope.

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