HPLC-ESI-MS characterization of phytochemicals in Serghina extract

Published: 19 January 2022| Version 3 | DOI: 10.17632/8z96bsx2pm.3
Yassine Oualcadi


HPLC analysis of serghina extract was performed on Thermo Fisher Scientific Dionex Ultimate 3000 HPLC System, equipped with the Ultimate 3000 RS Variable Wavelength UV DAD. DAD was performed in-line in UV–VIS spectra in the range of 200 to 800 nm. An Acclaim Polar Advantage column (5µm - C18 - 250 mm x 4.6 mm) was used at 30°C. Data acquisition was performed by Thermo Fisher Scientific Xcalibur software (version 3.1).


Steps to reproduce

The phase mobile consists of two solvents: 0.1% formic acid (Solvent A) and 0.25% acetonitrile-formic acid (60:40 v/v) (Solvent B). The gradient program was: 0 min, 100% A; 15 min, 80% A; 30 min, 70% A; 50 min, 100% B. The flow rate was 1.3 mL/min. The extract was subjected from HPLC to the Mass Spectrometer System (Thermo Scientific Orbitrap ID-X Tribrid). Temperature and capillary voltage were 300 ° C and 70 V, respectively. Full scan spectra were acquired in the range 100 - 1100 m/z in negative mode.


Universite Cadi Ayyad Ecole Normale Superieure de Marrakech


Mass Spectrometry, Phytochemicals, Reverse Phase High Performance Liquid Chromatography