Seurat Object: scRNA-seq of HSCs/HPCs under 5 oxygen tensions

Published: 26 February 2025| Version 1 | DOI: 10.17632/8zzm2nr4w6.1
Contributor:
James Ropa

Description

Single cell RNA-sequencing was performed on 4 umbilical cord blood units enriched for CD34+ cells, either expanded or unmanipulated. Two units were expanded under five different oxygen tensions (1%, 3%, 5%, 14%, and 21%) for two days, while two were expanded for seven days. For two units, we also sequenced unmanipulated (never cultured) CD34+ cells.

Files

Steps to reproduce

Details to reproduce the Seurat object used for all backend analysis are found in the R script uploaded here.

Institutions

  • Indiana University School of Medicine
    IN, Indianapolis

Categories

Cell Therapy, Oxygen, General Hematopoiesis, Umbilical Cord Blood Hematopoietic Stem Cell

Licence