Proteomic analyses to determine the impact of anti-FASII on Staphylococcus aureus protein expression in low stress conditions.

Published: 3 November 2019| Version 2 | DOI: 10.17632/9292c75797.2
Contributors:
Alexandra Gruss,
Gérald Kénanian,
Céline Henry,
jamila anba-mondoloni

Description

The associated study shows that Staphylococcus aureus can adapt to fatty acid synthesis inhibitors by incorporating exogenous fatty acids in their phospholipids. Our results show that bacteria adapt in low stress medium more readily than in high stress medium. We compared two environments, i- high membrane stress and ii- low membrane stress, and followed responses of strain USA300-FPR3757 spa to the biocide triclosan. High membrane stress medium contains free fatty acids. Low membrane stress medium is supplemented with serum. Strains are grown in BHI medium ("BHI"), BHI containing 3 fatty acids C14, C16, and C18:1 ("FA"), FA medium with the biocide triclosan ("FA-Tric"), FA medium supplemented with serum ("SerFA"), and SerFA supplemented with triclosan (SerFA-Tric). BHI, FA, and SFA cultures are harvested at OD600=1. FA-Tric and SerFA-Tric cultures are harvested at 2 hours and 4 hours. The data show protein reads corresponding to each growth condition.

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