Data regarding anti-quorum sensing and antimicrobial activity of Melaleuca alternifolia and Salvia sclarea essential oil against Pseudomonas aeruginosa

Published: 9 January 2023| Version 1 | DOI: 10.17632/94x7pjvxvw.1
Contributors:
Anmol Srivastava,
vivek kumar,
Deepmala Sharma,
Vishnu Agarwal

Description

Researchers are now looking for alternatives that reduce the virulence and pathogenic potential of the bacteria without killing it. It can be accomplished by interfering with the quorum sensing (QS) system of bacteria. In this article, we aim to determine the antimicrobial and anti-QS activity of Salvia sclarea and Melaleuca alternifolia essential oils. The sub-lethal concentration of these essential oils (EOs) was found with the help of a growth curve, and further experiments were carried out below this concentration. To check for their anti-quorum activity, a bioreporter strain E. coli pJN105LpSC11 (to measure the concentration of 3-oxo-C12-HSL) and Chromobacterium violaceum CV026 (to check for the reduction in the formation of violacein pigment) was used. Several virulence phenotype assays like pyocyanin, alginate, and protease production, along with swarming motility, were done. The effect of these essential oils on biofilm formation was also checked. The results were confirmed by checking the expression of genes by real-time PCR.

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Institutions

Motilal Nehru National Institute of Technology

Categories

Microbiology

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