NGS analysis of HTT editing in vitro and in vivo

Published: 22-08-2017| Version 3 | DOI: 10.17632/97zwpbfzdm.3
Contributors:
Nicolas Merienne,
Gabriel Vachey,
Nicole Déglon

Description

Tables S2-S3: Next-generation sequencing (NGS; MiSeq) analysis of on-target editing evaluating deletions and insertions (indels) in the CRISPR-HTT group in vitro and in vivo. For each experiment (in vitro and in vivo), the dataset is composed of an excel file giving for each read, the length, the alignment score (to the reference sequence), the CIGAR string and whether indels were detected closed to expected cleavage site or not. Tables S4-S5: Potential off-target sites for sgHTT and sgCas9 were assessed with the Bioconductor package CRISPRseek with default parameters. The tables report the potential off-targets for each sgRNA with a score equal or above 1.

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