CHIR99021 titration in 384-well plates of human pluripotent stem cells - analysis of nephron segment differentiation
Published: 9 July 2018| Version 1 | DOI: 10.17632/988tyf4fh8.1
Automated analysis of plates generated by liquid handling robots, in which human pluripotent stem cells were differentiated with concentrations of CHIR99021 ranging from 0 to 20 µM. Nephron segments (proximal tubules, distal tubules, and podocytes) were identified as a basis for identifying the organoids. See associated paper for details.
Steps to reproduce
Raw data were obtained using GE imaging instrumentation and analysis software. See associated paper for details.
University of Washington
High Throughput Analysis, Organoid