CHIR99021 titration in 384-well plates of human pluripotent stem cells - analysis of nephron segment differentiation

Published: 9 July 2018| Version 1 | DOI: 10.17632/988tyf4fh8.1
Benjamin Freedman


Automated analysis of plates generated by liquid handling robots, in which human pluripotent stem cells were differentiated with concentrations of CHIR99021 ranging from 0 to 20 µM. Nephron segments (proximal tubules, distal tubules, and podocytes) were identified as a basis for identifying the organoids. See associated paper for details.


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Raw data were obtained using GE imaging instrumentation and analysis software. See associated paper for details.