CHIR99021 titration in 384-well plates of human pluripotent stem cells - analysis of nephron segment differentiation

Published: 9 July 2018| Version 1 | DOI: 10.17632/988tyf4fh8.1
Contributor:
Benjamin Freedman

Description

Automated analysis of plates generated by liquid handling robots, in which human pluripotent stem cells were differentiated with concentrations of CHIR99021 ranging from 0 to 20 µM. Nephron segments (proximal tubules, distal tubules, and podocytes) were identified as a basis for identifying the organoids. See associated paper for details.

Files

Steps to reproduce

Raw data were obtained using GE imaging instrumentation and analysis software. See associated paper for details.

Institutions

University of Washington

Categories

High Throughput Analysis, Organoid

Related Links

Article
https://www.cell.com/cell-stem-cell/abstract/S1934-5909(18)30216-9
is source of this dataset

Licence