Antifungal activity of some Mentha species
Antifungal activity The bioassay was performed in Petri dishes (90x15 mm and 150x20 mm) with dissolving depending on mint 200, 300 and 400 µg/mL (Tween 20, 0.1%) of EO in previously sterilised Potato Dextrose Agar (PDA-Pronodisa) growth medium flasks at 45-50ºC, while the medium was still liquid to be distributed in Petri dishes. Petri dishes were inoculated with an 8 mm-diameter disk of a 7-day old colony on the PDA of each tested fungi. Plates were incubated in the dark at 25ºC for 7 days. Fungal growth was evaluated by measuring the colony diameter in two perpendicular directions daily. Five replicate dishes were used for each EO, dose and fungus, it was done 3 times. The control Petri dishes contained only PDA/Tween 20 (0.1%) and the analysed fungus. Mycelial growth was evaluated by measuring the perpendicular colony diameters after 7 days.