Antifungal activity of some Mentha species
Description
Antifungal activity The bioassay was performed in Petri dishes (90x15 mm and 150x20 mm) with dissolving depending on mint 200, 300 and 400 µg/mL (Tween 20, 0.1%) of EO in previously sterilised Potato Dextrose Agar (PDA-Pronodisa) growth medium flasks at 45-50ºC, while the medium was still liquid to be distributed in Petri dishes. Petri dishes were inoculated with an 8 mm-diameter disk of a 7-day old colony on the PDA of each tested fungi. Plates were incubated in the dark at 25ºC for 7 days. Fungal growth was evaluated by measuring the colony diameter in two perpendicular directions daily. Five replicate dishes were used for each EO, dose and fungus, it was done 3 times. The control Petri dishes contained only PDA/Tween 20 (0.1%) and the analysed fungus. Mycelial growth was evaluated by measuring the perpendicular colony diameters after 7 days.