Dataset of cell geometry quantification
Description
Quantification of cell geometry
Files
Steps to reproduce
Immunofluorescence images were captured using an Olympus FV3000 confocal microscope with a 60X oil immersion objective (NA 1.4). Cell shape analysis was conducted based on the immunofluorescence images using Fiji ImageJ. The cell contours used for cell shape analysis, were labeled with phalloidin-TRITC (cat.#1951, 1:1000 dilution). Cell height was calculated as the absolute difference between the upper edge (the position average of the highest point and the cell‒cell contact points on the apical surface) and the lower edge (the stress fibers) of the cell. The cell area and the lengths of the major and minor axes of the ellipse that fit the cell contour were directly calculated using the “Shape Descriptors” measurement function in Fiji ImageJ based on the transverse sectional view in the middle of the cell. Cell width is defined as the average of the major and minor axes. Cell orientation was determined by measuring the angle of the major axis of the ellipse. Cell shape analysis was performed by randomly selecting 60~150 cells from at least three independent experiments.
Institutions
- National Cheng Kung University
- National Cheng Kung University Department of Physiology