scDART-seq reveals distinct m6A signatures and mRNA methylation heterogeneity in single cells

Published: 17 January 2022| Version 1 | DOI: 10.17632/99cjyffbyc.1
Contributor:
Matthew Tegowski

Description

Stable cell lines with a doxycycline-inducible APOBEC1-YTH or APOBEC1-YTHmut transgene were treated with 1µg/mL doxycycline and cultured at 37 degrees C for 0 h or 24 h. Fluorescent images were captured using a Leica DMi8 inverted microscope (Excitation 470/40, Emission 525/50) using the LAS X software.

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Steps to reproduce

Stable cell lines with a doxycycline-inducible APOBEC1-YTH or APOBEC1-YTHmut transgene were treated with 1µg/mL doxycycline and cultured at 37 degrees C for 0 h or 24 h. Fluorescent images were captured using a Leica DMi8 inverted microscope (Excitation 470/40, Emission 525/50) using the LAS X software.

Institutions

Duke University

Categories

Transgene, Fluorescence Imaging

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