Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons. Wimmer et al.
Published: 23 January 2023| Version 1 | DOI: 10.17632/99g7j7rz7r.1
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Original gel images for: Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons. Figure 7B: CRISPR-dependent transposition and determination of transposon ends and insertion distance using the TXTL-based transposition assay with RoCAST. Figure 7C: CRISPR-dependent transposition in TXTL. Figure 7D: CRISPR-independent transposition in TXTL. Figure 7E: CRISPR-dependent transposition in E. coli. Figure 7F: CRISPR-independent transposition in E. coli. Figure S3E: Colony PCR of representative colonies associated with the CAA or AAA PAM targets. Figure S5C: CRISPR-dependent transposition with the AvCAST in TXTL. Figure S6A: CRISPR-dependent transposition with the V. cholerae CRISPR transposon (VcCAST) in TXTL.
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