Phylogeny_of_foetal_haematopoiesis_2020_LCM_images

Published: 26-11-2020| Version 1 | DOI: 10.17632/9b264dw38s.1
Contributor:
Mike Spencer Chapman

Description

These are the histological images to accompany the manuscript "Lineage tracing of human embryonic development and foetal haematopoiesis through somatic mutations" (bioRxiv 2020.05.29.088765; doi: https://doi.org/10.1101/2020.05.29.088765). The laser capture micro-dissection (LCM) images were taken from the camera on the laser-capture microdissection system (Leica LMD7) at 20-40x magnification. In most cases there are images from before and after LCM to illustrate the precise area cut and subjected to targeted or whole-genome sequencing. The slide scanner images (ending .ndpi) are high-resolution histology images taken with a Hamamatsu slide scanner, and should be viewed together with the corresponding annotation file (ending .ndpa) which highlights the areas undergoing LCM, and the corresponding unique identifier. They can be viewed using freely available software NDP.view (available at https://www.ndpserve.com/ndp/serve/home). The tissues are 5-10um thick and are from two human foetuses of 8- and 18-pcw weeks as described in manuscript. For the 8-pcw foetus there are images taken from epidermis, connective tissue of the limb, inter-vertebral discs, kidney, gut, heart, blood from inside the chambers of the heart and placenta. For the 18-pcw foetus there are images from the epidermis, hair follicles, peripheral nerves, heart and kidney.

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