De Novo Transcriptome Assembly and Data for the Blue-winged Teal (Anas discors)

Published: 15-11-2019| Version 1 | DOI: 10.17632/9bzr2sykrs.1
Contributors:
Amanda Dolinski,
Jarred Homola,
Jen Owen,
Mark Jankowski

Description

The blue-winged teal (Anas discors) is a recreationally and ecologically important dabbling duck species in North America. Transcriptomic data of this species can be used in public and animal health studies given its role as a natural reservoir host for avian influenza, which can be a zoonotic disease of high concern. Ileum and bursa of Fabricius tissues were sampled from six captive raised blue-winged teals, four of the six who were experimentally infected with low-pathogenic avian influenza virus H5N9. RNAseq data were generated from extracted total mRNA from each tissue and pooled to create a de novo assembly of the transcriptome using Trinity. A total of 571,105 transcripts were identified at 449,956 unique unigenes that have been functionally annotated. This transcriptome will be useful for future blue-winged teal gene expression research, especially in hypothesis driven differential expression studies to determine the driving forces of avian influenza host-pathogen interactions, spatial distribution, and transmission.

Files

Steps to reproduce

Pipeline scripts found here: https://github.com/OwenLabMSU/bwteDeNovo