A Comprehensive Dataset to Assess Morphological Changes Subsequent to Bleomycin Injury

Published: 15-12-2020| Version 1 | DOI: 10.17632/9djkffg5k5.1
Contributor:
Alessandro Venosa

Description

Mice were intratracheally instilled with either PBS or bleomycin (Venosa, 2020). 7 and 21 days following instillation, lungs were lavaged and left lungs inflation fixed with 2% paraformaldehyde. Lungs were dehydrated and paraffin embedded for sectioning. 5 micron sections were stained with hematoyxlin and eosin. Histology slides were scanned with the Olympus VS120 microscope at 400x. Slides were visualized on Olympus Olyvia software and ten high power field (400x) images were randomly chosen throughout the tissue. Care was taken to avoid large airways and field adjusted as needed. Images were then assessed in Image J to measure morphology. Morphological assessment was based on those commonly reported in the literature (Izbicki et al., 2002; Tourneux et al., 2009; Matute-Bello et al., 2011; Gilhodes et al., 2017). The scale was set and using the line tool, the alveolar wall width was measured. Alveolar walls were identified and measured in each field. Nuclei were also counted manually per field. Finally, the image type was changed to 8-bit, threshold set, and then the image converted to binary. The background (airspace) was quantified by creating a selection and measurement made. The total field measurement was used as the denominator to quantify the percent airspace.

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This is a method's dataset. Please refer to description above for detailed methodology.